FGA Knockout cell line(Hep G2)

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FGA Knockout cell line(Hep G2) Order

Catalog#

YKO-H425

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FGA Knockout cell line (Hep G2)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

FGA

Gene ID

2243

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10% FBS

Accession

CVCL_E0U8(Cellosaurus)

Target region

Knockout region: Exon 5 (based on transcript FGA-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hep G2			STR Info (Cell bank) Cell Line:GS-HepG2
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	9	13		9	13
D7S820	10	10		10	10
D16S539	12	12		12	12
VWA	17	17		17	17
TH01	9	9		9	9
AMEL	X	Y		X	Y
TPOX	8	9		8	9
CSF1PO	10	11		10	11
D12S391	21	25
FGA	22	25
D2S1338	19	20
D21S11	29	31
D18S51	13	14
D8S1179	15	16
D3S1358	15	16
D6S1043	13	13
PENTAE	15	20
D19S433	15.2	15.2
PENTAD	9	13
D1S1656	11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FGA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FGA gene knockout FGA Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

FGA Knockout cell line(Hep G2)

Catalog#

YKO-H425

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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FGA Knockout cell line(Hep G2)

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